FLOW MICROFLUOROMETRIES
\flˈə͡ʊ mˌa͡ɪkɹə͡ʊflʊ͡əɹˈɒmətɹɪz], \flˈəʊ mˌaɪkɹəʊflʊəɹˈɒmətɹɪz], \f_l_ˈəʊ m_ˌaɪ_k_ɹ_əʊ_f_l_ʊə_ɹ_ˈɒ_m_ə_t_ɹ_ɪ_z]\
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Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Platidiam
- An inorganic water-soluble platinum complex. After undergoing hydrolysis, it reacts DNA produce both intra interstrand crosslinks. These crosslinks appear to impair replication and transcription of DNA. The cytotoxicity cisplatin correlates with cellular arrest in G2 phase cell cycle.